Instrumentation Quiz – CytologyStuff

Instrument used to heat and cool specimens:

Flow Cytometer

Spectrophotometer

Fluorometer

Thermocycler

Correct! Wrong!

This technique is about 20 years old; however automation is much more recent.

PCR

In Situ Hybridization

Gel Electrophoresis

Allele Specific Oligonucleotide

Correct! Wrong!

Which metals can be found in the block of a thermocycler?

Aluminum and Silver

Beryllium and Copper

Bronze and Chromium

Titanium and Lead

Correct! Wrong!

Currently there is no software available for the analysis of FISH specimens.

True

False

Correct! Wrong!

A power supply is necessary to perform gel electrophoresis.

Please select 2 correct answers

True

False

Correct! Wrong!

Real Time PCR instruments measure

the amount of free radicals present after fluorescence.

the movement of DNA thru a gel matrix.

the activity level of viral particles.

the kinetics of product accumulation in each tube.

Correct! Wrong!

A microplate reader is able to read all but this:

Luminescence

Effervescence

Absorbance

Fluorescence

Correct! Wrong!

Which of the following alterations can be detected with FISH?

Duplication

Deletion

Translocation

All of the above

Correct! Wrong!

Thermocyclers are large pieces of equipment and require floor space in the lab.

True

False

Correct! Wrong!

Automation in the lab has the following benefits:

Reduction of human error

Increased throughput

Better control of variables

All of the above

Correct! Wrong!